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Scripts for

Wang, X., ... Heller, R. (2025). Population structure and domestication history of the Javan banteng.

Evaluation of assembly

Scripts to evaluate assembly.

Mapping

A pipeline for mapping and post-mapping filtering using PALEOMIX BAM pipeline.

Sample filtering

Script to assess post-mortem damage in historical samples using mapDamage2;

A pipeline designed to remove problematic samples prior to downstream analyses;

Script to perform a second and more sensitive analysis of relatedness within each sampling location using NgsRelate.

Sites filtering

A pipeline designed to avoid biases from low-quality mapping sites.

Genotype likelihood calculation

A pipeline designed to call genotype likelihood for output as BEAGLE.

Genotype calling, imputation and phasing

A pipeline designed to call genotypes;

Scripts to imputate and phase genotypes.

Population structure and admixture analyses

Script to infer principal component analysis (PCA) using HaploNet;

Script to estimate admixture proportions for each individual using NGSadmix;

Script to use evalAdmix.

Genetic diversity, divergence and inbreeding

Script to calculate genome-wide heterozygosity;

Script to infer Pairwise global Fst between each pair of populations;

Script to infer runs of homozygosity (ROH);

Script to calculate genome-wide heterozygosity without ROH.

Population history and introgression

Script to infer the population tree assuming different numbers of admixture events using TreeMix;

Script to infer the ancient admixture events, we calculated D statistics (ABBA-BABA) using the ANGSD;

Script to infer the f3-outgroup using the R package ADMIXTOOLS2.

Demographic history

Script to infer effective population size changes using PSMC;

Script to estimate domestication event between wild (Banteng-WildBorn) and domesticated (Bali-Bali) using Fastsimcoal2;

Script to estimate Confidence interval using Fastsimcoal2;

Script to perform simulations and calculate their Fst and pi during revision;

All 14 demographic model design for estimating domestication event by Fastsimcoal2.

Genome-wide selection scans

Script to perform sliding window Fst, nucleotide diversity and Tajima’s D;

Script to infer linkage disequilibrium by ngsLD;

Script to infer XP-EHH (Sabhrina).

Sabhrina_Genetic load

Scripts to infer genetic load.

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Evolutionary Genomics and Domestication of the Endangered Java Bantengs

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