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@DS3-2025

DS3 2025

Course Materials for Data Science for Developing Scholars in Down Syndrome Research (DS3) 2025

Main course website: https://includeds3.org/

The Data Science for Developing Scholars in Down Syndrome Research (DS3) course is a training program working to increase data sciences expertise across the INCLUDE Project and the Down syndrome research community.

Getting started with R and RStudio

Slides
GitHub repository: installing_updating_R-RStudio

Alternatives to running locally:
Posit Cloud
Cavatica
Apply for INCLUDE Cloud Credits

Introduction to the Tidyverse

Slides
GitHub repository: Rproject_template
GitHub repository: tidy_data_exercise

Morning sessions: Short Read Sequencing Workshop

Website: https://biodatasci.colorado.edu/shortread/
GitHub: Dowell-Lab/srworkshop

Afternoon sessions

Github (this site): DS3-2025

Day 1: The Human Trisome Project

Instructor: Matthew Galbraith
An overview of the Human Trisome Project and the journey from data to discovery.
Slides

Day 2: Omics and Data Science Concepts

Instructor: Matthew Galbraith
An introduction to important concepts in omics and best practices in data science.
Slides

Day 3: Reproducible data analysis using R and RStudio

Instructor: Matthew Galbraith
A guide to conducting reproducible data analysis in R using RStudio and tidy principles.
Slides
GitHub repository: Rproject_template

Day 4: Data Cleaning and Exploratory Data Analysis

Instructor: Matthew Galbraith
Exercises in data cleaning/wrangling in R using tidyverse principles and tools.
GitHub repository: intro_exploratory_data_analysis
GitHub repository: tidy_data_exercise

Day 5: Intro to Linear Regression and Hypothesis Testing in R

Instructor: Matthew Galbraith
An introduction to statistical modeling and testing in R
Slides
GitHub repository: linear_regression_exercise
GitHub repository: HTP_linear_regression_example

Day 6: Advanced Data Visualization and Analysis in R

Instructor: Matthew Galbraith
Examples and discussion of effective data visualization using ggplot in R.
Slides
GitHub repository: HTP_linear_regression_example
GitHub repository: HTP_DESeq2_analysis
GitHub repository: HTP_single_cell_dataviz_mass_cytometry

Day 7: Advanced Data Visualization and Analysis in R

Instructor: Jim Costello
Applications of simple machine learning methods to predict outcomes leveraging HTP datasets.

GitHub repository: Visualize_Cluster_HTP

Day 8: Supervised Machine Learning with HTP examples

Instructor: Jim Costello
Applications of simple machine learning methods to predict outcomes leveraging HTP datasets.

GitHub repository: PredictiveModels

Day 9: Analysis of INCLUDE data with R using Cavatica

Instructor: Matthew Galbraith and Velsera
Slides
A guide to locating data in the INCLUDE Data Hub and transfering to the Cavatica platform for analysis.
Apply for INCLUDE Cloud Credits
GitHub repository: HTP_linear_regression_Cavatica

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