diff --git a/emscripten.py b/emscripten.py
index 935d8212e11d8..274975c50dafe 100644
--- a/emscripten.py
+++ b/emscripten.py
@@ -886,7 +886,8 @@ def create_wasm64_wrappers(metadata):
     '_emscripten_dlsync_self_async': '_p',
     '_emscripten_proxy_dlsync_async': '_pp',
     '_wasmfs_rmdir': '_p',
-    '_wasmfs_unlink': '_p'
+    '_wasmfs_unlink': '_p',
+    'emscripten_wasm_worker_initialize': '_p_',
   }
 
   wasm64_wrappers = '''
diff --git a/src/library_sigs.js b/src/library_sigs.js
index 9fdbf18790fe1..8e69e8e218458 100644
--- a/src/library_sigs.js
+++ b/src/library_sigs.js
@@ -438,6 +438,8 @@ sigs = {
   emscripten_console_log__sig: 'vp',
   emscripten_console_warn__sig: 'vp',
   emscripten_create_audio_context__sig: 'ip',
+  emscripten_create_wasm_audio_worklet_node__sig: 'iipppp',
+  emscripten_create_wasm_audio_worklet_processor_async__sig: 'vippp',
   emscripten_create_worker__sig: 'ip',
   emscripten_current_thread_is_audio_worklet__sig: 'i',
   emscripten_date_now__sig: 'd',
@@ -621,6 +623,7 @@ sigs = {
   emscripten_stack_snapshot__sig: 'p',
   emscripten_stack_unwind_buffer__sig: 'ippi',
   emscripten_start_fetch__sig: 'vp',
+  emscripten_start_wasm_audio_worklet_thread_async__sig: 'vipipp',
   emscripten_supports_offscreencanvas__sig: 'i',
   emscripten_throw_number__sig: 'vd',
   emscripten_throw_string__sig: 'vp',
diff --git a/src/library_webaudio.js b/src/library_webaudio.js
index d0e6da3075404..9b4942f0aa472 100644
--- a/src/library_webaudio.js
+++ b/src/library_webaudio.js
@@ -126,9 +126,6 @@ let LibraryWebAudio = {
     'wasm_workers_id',
     '$_EmAudioDispatchProcessorCallback'],
   emscripten_start_wasm_audio_worklet_thread_async: function(contextHandle, stackLowestAddress, stackSize, callback, userData) {
-#if !AUDIO_WORKLET
-    abort('emscripten_create_wasm_audio_worklet() requires building with -s AUDIO_WORKLET=1 enabled!');
-#endif
 
 #if ASSERTIONS
     assert(contextHandle, `Called emscripten_start_wasm_audio_worklet_thread_async() with a null Web Audio Context handle!`);
@@ -156,7 +153,7 @@ let LibraryWebAudio = {
 #if WEBAUDIO_DEBUG
       console.error(`emscripten_start_wasm_audio_worklet_thread_async() addModule() failed!`);
 #endif
-      {{{ makeDynCall('viii', 'callback') }}}(contextHandle, 0/*EM_FALSE*/, userData);
+      {{{ makeDynCall('viip', 'callback') }}}(contextHandle, 0/*EM_FALSE*/, userData);
     };
 
     // Does browser not support AudioWorklets?
diff --git a/test/test_browser.py b/test/test_browser.py
index 36e2e38825569..3cb8a2ff790c9 100644
--- a/test/test_browser.py
+++ b/test/test_browser.py
@@ -418,7 +418,7 @@ def make_main_two_files(path1, path2, nonexistingpath):
 
   # Tests that user .html shell files can manually download .data files created with --preload-file cmdline.
   @parameterized({
-    'default': ([],),
+    '': ([],),
     'pthreads': (['-pthread', '-sPROXY_TO_PTHREAD', '-sEXIT_RUNTIME'],),
   })
   @requires_threads
@@ -5502,7 +5502,8 @@ def test_full_js_library_strict(self):
 
   # Tests the AudioWorklet demo
   @parameterized({
-    'default': ([],),
+    '': ([],),
+    'memory64': (['-sMEMORY64', '-Wno-experimental'],),
     'with_fs': (['--preload-file', test_file('hello_world.c') + '@/'],),
     'closure': (['--closure', '1', '-Oz'],),
     'asyncify': (['-sASYNCIFY'],),
@@ -5512,11 +5513,13 @@ def test_full_js_library_strict(self):
     'minimal_runtime_pthreads_and_closure': (['-sMINIMAL_RUNTIME', '-pthread', '--closure', '1', '-Oz'],),
   })
   def test_audio_worklet(self, args):
+    if '-sMEMORY64' in args and is_firefox():
+      self.skipTest('https://github.com/emscripten-core/emscripten/issues/19161')
     self.btest_exit(test_file('webaudio/audioworklet.c'), args=['-sAUDIO_WORKLET', '-sWASM_WORKERS'] + args)
 
   # Tests that posting functions between the main thread and the audioworklet thread works
   @parameterized({
-    'default': ([],),
+    '': ([],),
     'closure': (['--closure', '1', '-Oz'],),
   })
   def test_audio_worklet_post_function(self, args):
diff --git a/tools/gen_sig_info.py b/tools/gen_sig_info.py
index b0a9e85c2da45..e1a653c333dd1 100755
--- a/tools/gen_sig_info.py
+++ b/tools/gen_sig_info.py
@@ -231,6 +231,8 @@ def extract_sigs(symbols, obj_file):
 def extract_sig_info(sig_info, extra_settings=None, extra_cflags=None):
   tempfiles = shared.get_temp_files()
   settings = {
+    # Enable as many settings as we can here to ensure the maximum number
+    # of JS symbols are included.
     'STACK_OVERFLOW_CHECK': 1,
     'MAX_WEBGL_VERSION': 2,
     'FULL_ES3': 1,
@@ -238,6 +240,8 @@ def extract_sig_info(sig_info, extra_settings=None, extra_cflags=None):
     'FETCH': 1,
     'PTHREADS': 1,
     'SHARED_MEMORY': 1,
+    'AUDIO_WORKLET': 1,
+    'WASM_WORKERS': 1,
     # Currently GLFW symbols have different sigs for the same symbol because the
     # signatures changed between v2 and v3, so for now we continue to maintain
     # them by hand.